Most of the data manipulation attacks on deep neural networks (DNNs) during the training stage introduce a perceptible noise that can be catered by preprocessing during inference or can be identified during the validation phase. Therefore, data poisoning attacks during inference (e.g., adversarial attacks) are becoming more popular. However, many of them do not consider the imperceptibility factor in their optimization algorithms, and can be detected by correlation and structural similarity analysis, or noticeable (e.g., by humans) in a multi-level security system. Moreover, the majority of the inference attack relies on some knowledge about the training dataset. In this paper, we propose a novel methodology which automatically generates imperceptible attack images by using the back-propagation algorithm on pre-trained DNNs, without requiring any information about the training dataset (i.e., completely training data-unaware). We present a case study on traffic sign detection using the VGGNet trained on the German Traffic Sign Recognition Benchmarks dataset in an autonomous driving use case. Our results demonstrate that the generated attack images successfully perform misclassification while remaining imperceptible in both "subjective" and "objective" quality tests.