In this paper, we study the adversarial robustness of subspace learning problems. Different from the assumptions made in existing work on robust subspace learning where data samples are contaminated by gross sparse outliers or small dense noises, we consider a more powerful adversary who can first observe the data matrix and then intentionally modify the whole data matrix. We first characterize the optimal rank-one attack strategy that maximizes the subspace distance between the subspace learned from the original data matrix and that learned from the modified data matrix. We then generalize the study to the scenario without the rank constraint and characterize the corresponding optimal attack strategy. Our analysis shows that the optimal strategies depend on the singular values of the original data matrix and the adversary's energy budget. Finally, we provide numerical experiments and practical applications to demonstrate the efficiency of the attack strategies.