In this study an Artificial Neural Network was trained to classify musical instruments, using audio samples transformed to the frequency domain. Different features of the sound, in both time and frequency domain, were analyzed and compared in relation to how much information that could be derived from that limited data. The study concluded that in comparison with the base experiment, that had an accuracy of 93.5%, using the attack only resulted in 80.2% and the initial 100 Hz in 64.2%.